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Principles of Inheritance and Variation

Principles of Inheritance and VariationNEET Botany · Class 12 · NCERT Chapter 2

10 interactive concept widgets for Principles of Inheritance and Variation. Drag any slider, change any number, and watch the formula and the answer update live. Built so you understand how each NEET problem actually works, not just the final number.

Punnett square: monohybrid cross simulator

Pick parent genotypes and see all offspring with ratios. Includes preset crosses for monohybrid F1, F2, and the test cross.

Genetics

Punnett square: monohybrid cross simulator

Pick the genotypes of two parents (T = tall, dominant; t = dwarf, recessive). The Punnett square shows all possible offspring genotypes with their ratios.

Parent 1 (mother): Tt

TT
Tt
tt

Heterozygous (carrier)

Parent 2 (father): Tt

TT
Tt
tt

Heterozygous (carrier)

Tt × Tt

T
t
T

TT

Tall

Tt

Tall

t

Tt

Tall

tt

Dwarf

Genotypic ratio

1 TT : 2 Tt : 1 tt

(out of 4)

Phenotypic ratio

3 Tall : 1 Dwarf

(out of 4)

Try these classic crosses

P x P (homozygous dom x recessive)
F1 x F1 (heterozygous self) → 3:1
TEST CROSS → 1:1
Pure tall x pure tall

NEET key facts

!

F1 of TT x tt = all Tt (heterozygous, all show DOMINANT phenotype).

!

F2 of Tt x Tt = 1 TT : 2 Tt : 1 tt (genotypic) and 3 tall : 1 dwarf (phenotypic) = 3:1.

!

TEST CROSS (Tt x tt): if heterozygous → 1:1 ratio. If homozygous (TT x tt) → all tall.

!

Mendel's laws: Dominance + Segregation (from monohybrid). Independent Assortment (from dihybrid).

Try this

  • Try Tt × Tt: see the classic 3:1 phenotypic ratio (Mendel's monohybrid F2). Genotypic is 1:2:1.
  • Try Tt × tt (TEST CROSS): notice the 1:1 ratio - this is how Mendel verified the heterozygous genotype.
  • Try TT × tt: F1 - all Tt. All offspring look tall but are HETEROZYGOUS. This is why heterozygotes are sometimes called "carriers".

Dihybrid cross: 9:3:3:1

Mendel's classic dihybrid cross with full 4x4 Punnett square. Visualise round/wrinkled and yellow/green seed combinations.

Genetics

Dihybrid cross: Mendel's classic 9:3:3:1

Mendel's classic dihybrid cross with pea (round/wrinkled, yellow/green seeds). The F2 generation from RrYy x RrYy gives the famous 9:3:3:1 phenotypic ratio.

F1 self-cross: RrYy × RrYy

R = round (dominant), r = wrinkled; Y = yellow (dominant), y = green

Show 4x4 grid
Hide grid
RY
Ry
rY
ry
RY

RRYY

Round
Yellow

RRYy

Round
Yellow

RrYY

Round
Yellow

RrYy

Round
Yellow

Ry

RRyY

Round
Yellow

RRyy

Round
Green

RryY

Round
Yellow

Rryy

Round
Green

rY

rRYY

Round
Yellow

rRYy

Round
Yellow

rrYY

Wrinkled
Yellow

rrYy

Wrinkled
Yellow

ry

rRyY

Round
Yellow

rRyy

Round
Green

rryY

Wrinkled
Yellow

rryy

Wrinkled
Green

Phenotypic ratio (the famous 9:3:3:1)

Round Yellow

9/16

Both dominant; parental type

Round Green

3/16

Round dom + green rec; recombinant

Wrinkled Yellow

3/16

Wrinkled rec + yellow dom; recombinant

Wrinkled Green

1/16

Both recessive; parental type

9 : 3 : 3 : 1

(Round-Yellow : Round-Green : Wrinkled-Yellow : Wrinkled-Green)

Why 9:3:3:1?

The 9:3:3:1 ratio is the PRODUCT of two independent 3:1 ratios (one for each gene). Mathematically:

(3:1) × (3:1) = 9:3:3:1

This works ONLY when the two genes are on DIFFERENT chromosomes (independent assortment). For LINKED genes, the ratio is different.

NEET key facts

!

Dihybrid F2 phenotypic ratio = 9:3:3:1.

!

Genotypic ratio = 1:2:1:2:4:2:1:2:1 (9 genotype classes - more complex).

!

Mendel's 3rd law: INDEPENDENT ASSORTMENT - alleles of different genes assort independently into gametes.

!

Test cross of RrYy x rryy gives 1:1:1:1 phenotypic ratio (4 gamete types revealed).

!

For LINKED genes, the 9:3:3:1 ratio fails. Linked genes show parental types more than recombinants.

Try this

  • Count the squares: 9 round-yellow, 3 round-green, 3 wrinkled-yellow, 1 wrinkled-green. This is the 9:3:3:1 ratio.
  • Notice ROUND-GREEN and WRINKLED-YELLOW are RECOMBINANT phenotypes - new combinations not seen in the parents.
  • NEW combinations only appear because Mendel's 3rd law (independent assortment) splits the alleles into all 4 gamete types in equal proportions.

Inheritance pattern classifier

Four major inheritance patterns: complete dominance, incomplete dominance, codominance, multiple alleles. Compare heterozygote phenotypes and F2 ratios.

Genetics

Inheritance pattern classifier

Four major inheritance patterns: complete dominance, incomplete dominance, codominance, and multiple alleles. Click each to compare heterozygotes, F2 ratios, and examples.

Complete Dominance
Incomplete Dominance
Codominance
Multiple Alleles

Complete Dominance

Heterozygote looks like the dominant parent

When two contrasting alleles are present, only the DOMINANT allele is expressed. The recessive allele is masked. Heterozygote is phenotypically identical to the homozygous dominant. Mendel's discoveries are based on this.

Heterozygote phenotype:

Same as dominant homozygote

F2 ratio:

F2 = 3:1 phenotypic; 1:2:1 genotypic

Classic examples:

Mendel's pea: tall x dwarf → tall heterozygote

Mendel's 7 traits in pea

Most simple Mendelian crosses

Quick comparison

Pattern
Heterozygote
F2 ratio
Complete dominance
= dominant homozygote
3:1 phenotypic
Incomplete dominance
Intermediate (new colour)
1:2:1 (= genotypic)
Codominance
Both phenotypes visible
1:2:1 (= genotypic)
Multiple alleles
3+ alleles in population
Up to 4 phenotypes

NEET key facts

!

Complete dominance: heterozygote = dominant homozygote. Mendel's pea (3:1).

!

Incomplete dominance: heterozygote = INTERMEDIATE (e.g., pink Mirabilis). 1:2:1 ratio.

!

Codominance: heterozygote = BOTH phenotypes (e.g., AB blood, roan coat). 1:2:1 ratio.

!

Multiple alleles: more than 2 alleles in population. ABO has 3 (IA, IB, i).

!

IA and IB are CODOMINANT to each other. Both are DOMINANT over i. So O blood = ii (recessive).

Try this

  • Compare INCOMPLETE DOMINANCE (Mirabilis pink) vs CODOMINANCE (AB blood). Both have 1:2:1 ratio but different heterozygote appearance.
  • Pink in Mirabilis = NEW colour (blended). AB blood = BOTH antigens (no blending; two distinct molecules side by side).
  • Multiple alleles: ABO has 3 alleles. Any individual has only 2 (one from each parent). The population has 3.

ABO blood group: multiple alleles + codominance

3 alleles (IA, IB, i) → 4 blood groups (A, B, AB, O). Pick parent genotypes and see possible children blood groups with reference table.

ABO Blood Group

ABO blood group: multiple alleles + codominance

The ABO system has 3 alleles (IA, IB, i) giving 4 blood groups (A, B, AB, O). IA and IB are codominant; i is recessive to both. Pick parents to see possible offspring blood groups.

The 3 alleles

IA

A antigen

IB

B antigen

i

No antigen

IA and IB are CODOMINANT to each other. Both DOMINANT over i.

Parent 1 (mother): IAi → Group

A

IAIA (A)
IAIB (AB)
IAi (A)
IBIB (B)
IBi (B)
ii (O)

Parent 2 (father): IBi → Group

B

IAIA (A)
IAIB (AB)
IAi (A)
IBIB (B)
IBi (B)
ii (O)

IAi × IBi

IB
i
IA

IAIB

Group AB

IAi

Group A

i

iIB

Group B

ii

Group O

Offspring blood group probabilities

A

1/4

= 25%

B

1/4

= 25%

AB

1/4

= 25%

O

1/4

= 25%

ABO blood group reference

Group
Genotypes
Antigens on RBC
Antibodies in plasma
Frequency in India
A
IA IA, IA i
A
Anti-B
~22%
B
IB IB, IB i
B
Anti-A
~33%
AB
IA IB
A + B (both)
No antibodies
~7%
O
ii
None (just H)
Both anti-A and anti-B
~38%

NEET key facts

!

ABO has 3 alleles: IA, IB, i. Multiple alleles example.

!

IA and IB CODOMINANT (both expressed in IA IB → AB blood). Both DOMINANT over i.

!

Genotype to phenotype: IA IA or IA i = A. IB IB or IB i = B. IA IB = AB. ii = O.

!

O is universal donor (no A or B antigens to react with recipient antibodies).

!

AB is universal recipient (no antibodies; can accept any blood type).

!

AB man + O woman → only A or B children possible. NEET classic problem.

Try this

  • Try IA IB (AB) × ii (O): the children are 2 IAi (A) + 2 IBi (B). NO AB or O children possible. Classic NEET problem.
  • Try IA i (A) × IB i (B): the children can be A, B, AB, OR O. Both parents heterozygous → all 4 groups possible.
  • Try IA IA (A pure) × IB IB (B pure): all children are IA IB (AB) - codominance gives the heterozygote phenotype.

Sex determination systems

Four systems across organisms: XY (humans), XO (grasshoppers), ZW (birds), ZO (some moths). Click each to see who is heterogametic.

Sex Determination

Sex determination systems across organisms

Four major chromosomal sex determination systems. The HETEROGAMETIC sex (which produces 2 types of gametes) determines the offspring sex. Click each to see who is heterogametic.

XY system (humans)
XO system (grasshopper)
ZW system (birds)
ZO system (some moths)

XY system (humans)

The classic mammal system. The Y chromosome carries male-determining genes (SRY in humans). Male is heterogametic. Father determines sex of children (X-bearing sperm = girl, Y-bearing = boy). 22 pairs of autosomes + XX or XY = 46 chromosomes in humans.

FEMALE

XX

MALE

XY

Heterogametic sex (determines offspring sex):

MALE (produces X and Y sperm)

Homogametic sex:

FEMALE (all eggs are X)

Examples:

Humans

Most mammals

Drosophila (fruit fly)

FEMALEXXMALEXY×Eggs:Sperm:XXXY↓ Possible offspring ↓XXFemaleXYMaleXXFemaleXYMale

NEET key facts

!

XY system (humans, Drosophila): male XY (heterogametic), female XX. SRY gene on Y determines maleness.

!

XO system (grasshopper): male XO (only ONE X, no Y), female XX. Males produce X-bearing and no-chromosome sperm.

!

ZW system (birds, butterflies): FEMALE ZW (heterogametic), male ZZ. Reverse of XY.

!

ZO system (some moths): female ZO, male ZZ.

!

In humans, the FATHER determines the child's sex (he provides X or Y). Probability of son = 50%.

!

NEET memory: XY = male heterogametic, ZW = female heterogametic.

Try this

  • Click "XY system": humans / mammals. The father determines sex (X sperm = girl, Y sperm = boy).
  • Click "ZW system": birds. Notice that the FEMALE is heterogametic - opposite of mammals.
  • Click "XO system": grasshoppers. Notice the male has only ONE X (and no Y at all). Males are heterogametic.

Sex-linked (X-linked) inheritance simulator

Why haemophilia and colour blindness are more common in males. Pick parent genotypes and see daughter / son outcomes.

Sex-Linked Inheritance

Sex-linked (X-linked) inheritance simulator

X-linked recessive traits like haemophilia and colour blindness are more common in males. Pick parents and see offspring outcomes for each sex.

Haemophilia
Colour blindness
DMD

X = normal allele. Xc = recessive disease allele (e.g., haemophilia). Y has no allele for this trait.

Mother: XcX (Carrier (heterozygous))

XX
XcX
XcXc

Father: XY (Normal)

XY
XcY
X
Y
Xc

XXc

CARRIER daughter

XcY

AFFECTED son

X

XX

Normal daughter

XY

Normal son

Why are males more affected by X-linked recessive traits?

Males have only ONE X chromosome (XY). If they inherit the recessive allele Xc, there is NO second X to mask it. So a single recessive allele is enough to cause the disease. Males are HEMIZYGOUS for X-linked genes.

Females have TWO X chromosomes (XX). They need TWO recessive alleles (XcXc) to express the disease. With one recessive allele (XcX), they are CARRIERS - they have the allele but do not show the disease.

NEET key facts

!

X-linked recessive: haemophilia, colour blindness, Duchenne muscular dystrophy (DMD).

!

Affected MALES have only ONE X (XcY) - hemizygous - single allele expresses.

!

CARRIER females are XcX (heterozygous) - phenotypically normal but pass the allele.

!

A father's X-linked allele is passed to ALL daughters (never to sons; sons get Y from father).

!

A carrier mother (XcX) x normal father (XY): 50% sons affected, 50% daughters carriers.

Try this

  • Try carrier mother (XcX) × normal father (XY): notice 1 in 4 daughters is carrier, 1 in 4 sons is affected. Classic Queen Victoria pedigree pattern.
  • Try affected mother (XcXc) × normal father (XY): ALL daughters become carriers (XcX), ALL sons become affected (XcY). Severe situation.
  • Try carrier mother × affected father (XcY): some daughters become affected (XcXc), some carriers (XcX); 50% sons affected.

Linkage and recombination calculator

Recombination frequency calculator. RF = recombinants / total × 100. Visualise linkage strength on a 0 to 50% scale.

Linkage

Linkage and recombination calculator

When two genes are on the same chromosome, they tend to be inherited together. Crossing over creates recombinants. The recombination frequency tells you how close the genes are.

Out of 100 test cross offspring, how many are PARENTAL (non-recombinant) types?

85

So 15 are recombinant types (new combinations).

Recombination frequency

15.0%

= 15.0 cM (centimorgans)

MEDIUM linkage

The formula

Recombination frequency = (recombinants / total) × 100

1 cM (centimorgan) = 1% recombination frequency.

MAXIMUM possible RF = 50% (genes effectively unlinked, on different chromosomes or very far apart).

Linkage strength scale

0 (perfect linkage)
10
25
50% (unlinked)

T.H. Morgan and Drosophila

Thomas Hunt Morgan and his students (Sturtevant, Bridges, Muller) discovered linkage in Drosophila melanogaster (fruit fly). They found that some genes did NOT assort independently as Mendel had predicted - they tended to be inherited together. They concluded these genes are on the SAME chromosome (linked). Crossing over during meiosis I separates linked genes, creating recombinants. Morgan won the Nobel Prize in 1933 for this work.

NEET key facts

!

Linked genes: located on the SAME chromosome. Tend to be inherited together.

!

Crossing over during meiosis I creates RECOMBINANT gametes.

!

Recombination frequency (RF) = (recombinants / total) × 100. Maximum = 50%.

!

1 cM (centimorgan) = 1% RF. Used to map gene positions on chromosomes.

!

Mendel's law of independent assortment WORKS only for genes on different chromosomes.

!

Morgan studied this in Drosophila. Sturtevant created the first genetic map.

Try this

  • Set parental = 100 (recombinant = 0): RF = 0%. The genes are PERFECTLY linked (no crossing over between them).
  • Set parental = 50 (recombinant = 50): RF = 50%. The genes behave INDEPENDENTLY (effectively unlinked - 9:3:3:1 ratio).
  • Set parental = 90 (recombinant = 10): RF = 10%. Tight linkage, genes are 10 cM apart.

Chromosomal disorders explorer

Five major aneuploidies: Down's, Klinefelter's, Turner's, Edward's, Patau. All caused by non-disjunction.

Chromosomal Disorders

Chromosomal disorders explorer

Five major chromosomal disorders. All caused by ANEUPLOIDY (abnormal chromosome number from non-disjunction). Click each to see karyotype, symptoms, and discovery.

Down's syndrome
Klinefelter's syndrome
Turner's syndrome
Edward's syndrome
Patau syndrome

Down's syndrome

Karyotype

Trisomy 21 (47, +21)

Total chromosomes

47

Type

Autosomal

Cause:

Extra copy of chromosome 21 (3 copies instead of 2). Caused by NON-DISJUNCTION of chromosome 21 during meiosis I or II.

Key symptoms / features:

Short stature

Characteristic facial features (small round face, flat nasal bridge, slanted eyes)

Intellectual disability (mild to moderate)

Congenital heart defects (~50% of cases)

Single deep transverse palm crease (simian crease)

Increased risk with maternal age over 35

Discovered: Langdon Down (1866) - clinical description; cause identified by Lejeune in 1959

What is non-disjunction?

NON-DISJUNCTION is the failure of homologous chromosomes (in meiosis I) or sister chromatids (in meiosis II) to separate properly. Result: one daughter cell gets an EXTRA chromosome (n+1 gamete) and another gets ONE FEWER (n-1 gamete). When these abnormal gametes fertilise normal ones, the zygote has extra (trisomy) or missing (monosomy) chromosomes.

ANEUPLOIDY = abnormal chromosome number. POLYPLOIDY = extra full chromosome SETS (common in plants, e.g., 4n).

NEET key facts

!

Down's syndrome = TRISOMY 21 (47 chromosomes). Autosomal aneuploidy. Risk increases with maternal age.

!

Klinefelter's syndrome = 47, XXY. Extra X in MALE. Sterile, tall, gynecomastia.

!

Turner's syndrome = 45, XO. MISSING X in female. Short, sterile, no secondary sexual characteristics.

!

Edward's syndrome = trisomy 18. Patau syndrome = trisomy 13. Both severe, usually fatal.

!

All caused by NON-DISJUNCTION (failure of chromosomes to separate during meiosis).

!

ANEUPLOIDY = abnormal number. POLYPLOIDY = extra full sets (4n, 6n in plants).

Try this

  • Click "Down's syndrome": notice it is AUTOSOMAL (chromosome 21, not sex). Karyotype 47.
  • Click "Klinefelter's" and "Turner's": both are SEX CHROMOSOMAL. Klinefelter has EXTRA X in male; Turner has MISSING X in female.
  • Compare karyotypes: Down 47, Klinefelter 47, Turner 45. The "47" disorders (Down, Klinefelter) have one EXTRA chromosome; Turner has one FEWER.

Pedigree analysis: 4 inheritance patterns

Standard pedigree symbols and decision tree. Identify autosomal vs X-linked, dominant vs recessive from family trees.

Pedigree

Pedigree analysis: 4 inheritance patterns

A pedigree is a family tree showing inheritance of a trait. Identifying the pattern (autosomal dominant, recessive, X-linked dominant, or recessive) helps determine genotypes and predict offspring.

Pedigree symbols

Unaffected male

Affected male

Unaffected female

Affected female

Carrier male (rare)

Carrier female

Marriage line

|

Parent-offspring line

Autosomal Dominant
Autosomal Recessive
X-Linked Recessive
X-Linked Dominant

Autosomal Recessive

Pedigree features:

May SKIP generations (carriers do not show the trait)

Often appears in offspring of unaffected parents (both carriers)

Both sexes equally affected

Increased frequency with consanguineous marriage

Inheritance rules:

Two unaffected carriers (Aa x Aa) have ~25% affected children

Two affected parents (aa x aa) have ALL affected children

Horizontal pattern in pedigree (siblings affected, parents not)

Examples:

Sickle cell anaemia

Cystic fibrosis

Phenylketonuria (PKU)

Albinism

Tay-Sachs disease

How to identify the pattern from a pedigree

  1. Does the trait SKIP generations? Yes → recessive. No → dominant.
  2. Are MALES disproportionately affected? Yes → X-linked recessive likely.
  3. Does the trait show FATHER-to-SON transmission? Yes → cannot be X-linked (must be autosomal or Y-linked).
  4. Are TWO UNAFFECTED parents producing affected children? Yes → recessive (they are carriers).
  5. Does an AFFECTED FATHER pass to ALL daughters but NO sons? → X-linked dominant.

NEET key facts

!

Pedigree symbols: square = male, circle = female, filled = affected, half-filled = carrier.

!

Autosomal recessive: skips generations, two carriers can have affected child. Sickle cell, PKU, albinism.

!

Autosomal dominant: appears in every generation. Marfan, Huntington's, polydactyly.

!

X-linked recessive: more in MALES, skips through carrier mothers. Haemophilia, colour blindness, DMD.

!

NEET trick: father-to-son transmission RULES OUT X-linked (sons get Y from father, not X).

Try this

  • If you see two unaffected parents with an affected child: it is RECESSIVE (parents are carriers).
  • If a trait appears in EVERY generation: it is likely DOMINANT.
  • If MALES are mostly affected and the trait skips generations: think X-LINKED RECESSIVE (haemophilia pattern).

Principles of Inheritance and Variation NEET quiz

12-question scored quiz covering Mendel, monohybrid / dihybrid crosses, ABO blood, sex determination, linkage, and chromosomal disorders.

Genetics

Principles of Inheritance and Variation: 12-question NEET quiz

One question at a time. Pick an option, see the explanation, then move to the next.

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Q1 / 12
Mendel

Mendel chose Pisum sativum (garden pea) primarily because:

A. Pea is the only plant with contrasting traits

B. Pea has true-breeding lines, contrasting traits, controllable pollination, and large progeny

C. Pea grows only in temperate climates

D. Pea has many chromosomes

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